Antioxidant and Anti-Diabetic Activities of the Methanolic Extract of Premna integrifolia Bark

Premna integrifolia Linn (Family: Verbenaceae) synonyms of Premna serratifolia has tremendous medicinal value. The methanolic extract of Premna integrifolia (MEPI) bark was subjected to investigate its antidiabetic activity along with its in vitro antioxidant activity. Oral glucose tolerance test (OGTT), normoglycemic test and alloxan induced diabetic test was consider for antidiabetic activity evaluation. In addition, total phenolic content, total antioxidant activity, scavenging of 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as reducing power assessment was used to evaluate antioxidant potentiality of MEPI. The continuous post treatment for 120 min with the MEPI showed potential hypoglycemic activity in OGTT and normoglycemic rats. At a dose of 300 mg/kg the extract, considerable drop in elevated blood glucose level was observed in the alloxan induced diabetic (p<0.05) rat after 7 days. It revealed radical scavenging properties in the DPPH assay (IC = 8.61 ± 0.16μg/ml). The finding of this study suggested that MEPI provide as a part of 50 scientific support for the use of this species in traditional medicine for Diabetes mellitus.